Factors Influencing the Prediction Accuracy of Model Peptides in Reversed-Phase Liquid Chromatography
نویسندگان
چکیده
Hydrophobicity is an important physicochemical property of peptides in solution. As well as being strongly associated with peptide stability and aggregation, hydrophobicity governs the solution based chromatographic separation processes, specifically reversed-phase liquid chromatography (RPLC). In addition, a major comparison to H-bonding, electrostatic, aromatic properties intermolecular interactions. However, wide range molecular factors can influence hydrophobicity, accurate predictions depending on specific amino acid compositions, structure, conformation. It noticeable that composition, position acid, its neighbouring groups play crucial role elution process. light this, same behaved differently chain. Extra attention should be paid denaturation process during course elution, it has been shown complicate alter pattern. This paper reports key and, consequently, RPLC behaviour peptides, will conclude by proposing improved prediction algorithms for RPLC.
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ژورنال
عنوان ژورنال: Separations
سال: 2023
ISSN: ['2297-8739']
DOI: https://doi.org/10.3390/separations10020081